RapidDNA: Transplant version combines the LIMS function with ChimerMarker® automated chimerism analysis software from SoftGenetics® This can be fully customised to fit your requirements. Our LIMS is specifically designed to automate DNA analysis workflow. Ecology/Agriculture/Wild life (SNPWave®/SNPlex®, AFLP, t-RFLP, Microsatellite (SSR, STR, VNTR), Kinship Analysis of Wild Populations and data base, SBE/ SnapShot®, TILLING® & EcoTilling with slabgel or capillary data, MLVA).Clinical research (Chimerism testing, Repeat Expansion Disorders, Fragile X, MLPA® (Multiplex Ligation‐dependent Probe Amplification), MS/MLPA®, Trisomy Analysis, Haplotype Analysis, Loss of Heterozygosity Analysis (LOH), ARMs & Comparative Analysis (Cystic Fibrosis Analysis), MSI (Microsatellite Instability) Analysis.Animal/Plant (Forensic plant identification, Forensic animal identification)Įxample of non-forensic applications include:.Military (Identification of soldiers lost in the battle, Military DNA “dog tags” – DNA identification for military personnel).Private lab (Legal paternity test, Ancestry test, Grand-Parentage test, Sibling DNA test, Animal DNA test).Immigration (Biological relationships determination for immigration purposes).Law enforcement (Crime samples, Missing persons).RapidDNA can be utilised for both forensic (legal) and non-forensic applications.Įxample of forensic applications include: STR, Y-STR profiles (Human, Animal, Plant, etc).Modular, scalable, secured and fully customisable.Provides ‘intuitive users interface’ for every step of the work.Fully customisable - applied to local jurisdictions & languages.Modular system - scalable to fit future needs.Users conveniently access the software via the graphical user interface (GUI) in their web browser from authorised client computers.Browser-based - Software installed only on a centralised server.Build up a DNA Database for storing DNA profiles that enables searching and comparing of DNA samples.Consists of LIMS specially designed to cover the whole DNA analysis workflow using STR markers or similar technology.A software package developed to automate DNA identification process and build up DNA Database.The system enables searching and comparing of DNA profiles per users requirements. It creates a centralised DNA database for storing DNA profiles of both known individuals and unknown (e.g., crime scene samples). Purified and quantified DNA from mock crime scene samples were used to demonstrate the utility of these cartridges in an established forensic laboratory.RapidDNA : Standard version combines LIMS with SQL Database capability. We report on sensitivity, reproducibility, concordance, DNA mixtures and carryover for EXT sample cartridges pre-loaded with GlobalFiler ® Express and AmpFLSTR ® NGM SElect™ Express, (Thermo Fisher Scientific, Waltham, MA) STR chemistries. Once the sample is loaded in the RapidHIT EXT sample cartridge, a DNA profile is produced after amplification, detection and automated data analysis. RapidHIT EXT sample cartridge allows a user to generate a forensic DNA profile from less than 250 pg of extracted and quantified DNA in less than 90 min with less than one-minute hands-on time. A new generation of the RapidHIT sample cartridge for RapidHIT ID has been designed and tested which allows the loading of extracted and quantified DNA. RapidHIT ® 200 and RapidHIT ID are rapid DNA systems developed by IntegenX (Pleasanton, CA) and validated for the use of buccal swabs. Rapid DNA Analysis is a hands-free “swab in – profile out” process which consists of automated DNA extraction, amplification, separation, detection, and allele calling without human intervention. However, PCR amplification and electrophoretic analysis remain largely manual. Routine DNA extraction and quantitation is readily available. Simplification and integration of the routine steps would dramatically improve turn-around time and reduce the risk of operator contamination. Crime scene DNA sample processing involves both complex and routine processing steps. The turn-around time of urgent crime scene DNA samples is often far longer than desired by law enforcement.
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